Job Description
DUTIES AND RESPONSIBILITIES
Responsible for purification of antibodies and recombinant proteins using AKTA FPLC and manual methods. Must execute and document work using SOPs and must follow strict GMP requirements under ISO 13485 standards. Coordinates preparation and transfer of purified antibody and protein samples to other functional teams.
Executes analytical testing of purified antibodies and proteins using SEC, SDS-PAGE, Western blot, MADLS, and qPCR.
Responsible for transfection and/or expression of antibodies and recombinant proteins from various mammalian cell lines, including but not limited to CHO and hybridomas as well as E. coli. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques
Responsible for the execution of experiments with varying complexity using advanced molecular biology techniques.
Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.
Involvement with scheduling, operational and maintenance duties required within the laboratory.
We are a company committed to creating diverse and inclusive environments where people can bring their full, authentic selves to work every day. We are an equal opportunity/affirmative action employer that believes everyone matters. Qualified candidates will receive consideration for employment regardless of their race, color, ethnicity, religion, sex (including pregnancy), sexual orientation, gender identity and expression, marital status, national origin, ancestry, genetic factors, age, disability, protected veteran status, military or uniformed service member status, or any other status or characteristic protected by applicable laws, regulations, and ordinances. If you need assistance and/or a reasonable accommodation due to a disability during the application or recruiting process, please send a request to HR@insightglobal.com.To learn more about how we collect, keep, and process your private information, please review Insight Global's Workforce Privacy Policy: https://insightglobal.com/workforce-privacy-policy/.
Required Skills & Experience
KNOWLEDGE AND SKILLS
Protein purification with liquid chromatography and quality assurance using various analytical methods.
Protein expression using various systems including but not limited to mammalian cells, insect cells, and bacteria.
Proficient with aseptic technique and cell-culture techniques is required.
Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
Effective written and oral communication skills.
Demonstrated ability to follow established policies and procedures.
Demonstrated ability to keep neat, accurate, and complete records.
Effective analytical and problem-solving skills.
EDUCATION AND EXPERIENCE
A Bachelor’s or Master of Science degree in biochemistry, cellular or molecular biology, immunology, or related discipline and a minimum of 2-4 years of laboratory experience required. Protein purification, protein expression, molecular biology and tissue culture experience required
Nice to Have Skills & Experience
Preferred: Familiarity with assay automation and automated liquid handlers.
Preferred: Experience with cell line engineering
Preferred: Hand-on experience with Flow Cytometry and the ability of analyzing Flow data is preferred.
Benefit packages for this role will start on the 1st day of employment and include medical, dental, and vision insurance, as well as HSA, FSA, and DCFSA account options, and 401k retirement account access with employer matching. Employees in this role are also entitled to paid sick leave and/or other paid time off as provided by applicable law.